p3 primary cell nucleofector solution buffer Search Results


nucleofectortm solution  (Amaxa)
86
Amaxa nucleofectortm solution
Nucleofectortm Solution, supplied by Amaxa, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/p3+primary+cell+nucleofector+solution+buffer/kit+neuron+nucleofector+rat/pm40992251-46-22-26
Average 86 stars, based on 1 article reviews
nucleofectortm solution - by Bioz Stars, 2026-10
86/100 stars
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amaxa 4d nucleofector  (Amaxa)
86
Amaxa amaxa 4d nucleofector
Amaxa 4d Nucleofector, supplied by Amaxa, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/p3+primary+cell+nucleofector+solution+buffer/4d+amaxa+nucleofector/pmc10277887-375-10-10
Average 86 stars, based on 1 article reviews
amaxa 4d nucleofector - by Bioz Stars, 2026-10
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p3 primary cell 4d nucleofector x kit  (Amaxa)
86
Amaxa p3 primary cell 4d nucleofector x kit
P3 Primary Cell 4d Nucleofector X Kit, supplied by Amaxa, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/p3+primary+cell+nucleofector+solution+buffer/electroporation/pmc12858050-66-5-15
Average 86 stars, based on 1 article reviews
p3 primary cell 4d nucleofector x kit - by Bioz Stars, 2026-10
86/100 stars
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synthetic modified sgrna  (Synthego Inc)
86
Synthego Inc synthetic modified sgrna
Synthetic Modified Sgrna, supplied by Synthego Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/p3+primary+cell+nucleofector+solution+buffer/modified+sgrna+synthetic/pmc08923057-508-7-10
Average 86 stars, based on 1 article reviews
synthetic modified sgrna - by Bioz Stars, 2026-10
86/100 stars
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sgrna  (Synthego Inc)
86
Synthego Inc sgrna
Targeted integration at the TCR locus and simultaneous KO of B2M. ( A ) Schematic of experimental design in primary T cells. An eGFP-expressing 4.4 kb cargo was integrated into the TRAC locus using an ssODN containing an attP site as an HDR template, together with a <t>sgRNA</t> targeting the TRAC locus. A B2M-targeting guide RNA can be co-delivered to achieve simultaneous KO at the B2M locus. Created in BioRender. Bassett, A. (2025) https://BioRender.com/lwtgerr . ( B ). Left: Bar chart showing ∼12% tagging efficiency at the TRAC locus in primary T cells. Data represent triplicate experiments; error bars indicate standard deviation. Data were analysed using two-sided t test. Right: Representative flow cytometry plot illustrating successful eGFP integration. ( C ) Schematic and flow cytometry analysis of simultaneous eGFP integration at the TRAC locus and B2M KO in primary T cells. All components were delivered in a <t>single</t> <t>nucleofection.</t> Seven days post-nucleofection, flow cytometry analysis revealed that >90% of eGFP-positive cells (indicating successful TRAC targeting) lacked B2M expression, compared to ∼30% B2M KO in the total cell population. Error bars indicate standard deviation. Data were analysed using two-sided t test.
Sgrna, supplied by Synthego Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/p3+primary+cell+nucleofector+solution+buffer/sgrna/pmc12359036-133-2-3
Average 86 stars, based on 1 article reviews
sgrna - by Bioz Stars, 2026-10
86/100 stars
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amaxa p3 solution  (Amaxa)
86
Amaxa amaxa p3 solution
Targeted integration at the TCR locus and simultaneous KO of B2M. ( A ) Schematic of experimental design in primary T cells. An eGFP-expressing 4.4 kb cargo was integrated into the TRAC locus using an ssODN containing an attP site as an HDR template, together with a <t>sgRNA</t> targeting the TRAC locus. A B2M-targeting guide RNA can be co-delivered to achieve simultaneous KO at the B2M locus. Created in BioRender. Bassett, A. (2025) https://BioRender.com/lwtgerr . ( B ). Left: Bar chart showing ∼12% tagging efficiency at the TRAC locus in primary T cells. Data represent triplicate experiments; error bars indicate standard deviation. Data were analysed using two-sided t test. Right: Representative flow cytometry plot illustrating successful eGFP integration. ( C ) Schematic and flow cytometry analysis of simultaneous eGFP integration at the TRAC locus and B2M KO in primary T cells. All components were delivered in a <t>single</t> <t>nucleofection.</t> Seven days post-nucleofection, flow cytometry analysis revealed that >90% of eGFP-positive cells (indicating successful TRAC targeting) lacked B2M expression, compared to ∼30% B2M KO in the total cell population. Error bars indicate standard deviation. Data were analysed using two-sided t test.
Amaxa P3 Solution, supplied by Amaxa, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/p3+primary+cell+nucleofector+solution+buffer/amaxa+p3+solution/pmc13025117-249-4-4
Average 86 stars, based on 1 article reviews
amaxa p3 solution - by Bioz Stars, 2026-10
86/100 stars
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pbs  (Caisson Labs)
86
Caisson Labs pbs
Targeted integration at the TCR locus and simultaneous KO of B2M. ( A ) Schematic of experimental design in primary T cells. An eGFP-expressing 4.4 kb cargo was integrated into the TRAC locus using an ssODN containing an attP site as an HDR template, together with a <t>sgRNA</t> targeting the TRAC locus. A B2M-targeting guide RNA can be co-delivered to achieve simultaneous KO at the B2M locus. Created in BioRender. Bassett, A. (2025) https://BioRender.com/lwtgerr . ( B ). Left: Bar chart showing ∼12% tagging efficiency at the TRAC locus in primary T cells. Data represent triplicate experiments; error bars indicate standard deviation. Data were analysed using two-sided t test. Right: Representative flow cytometry plot illustrating successful eGFP integration. ( C ) Schematic and flow cytometry analysis of simultaneous eGFP integration at the TRAC locus and B2M KO in primary T cells. All components were delivered in a <t>single</t> <t>nucleofection.</t> Seven days post-nucleofection, flow cytometry analysis revealed that >90% of eGFP-positive cells (indicating successful TRAC targeting) lacked B2M expression, compared to ∼30% B2M KO in the total cell population. Error bars indicate standard deviation. Data were analysed using two-sided t test.
Pbs, supplied by Caisson Labs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/p3+primary+cell+nucleofector+solution+buffer/pbs/pmc08792099-204-19-25
Average 86 stars, based on 1 article reviews
pbs - by Bioz Stars, 2026-10
86/100 stars
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gene targeting  (Synthego Inc)
86
Synthego Inc gene targeting
Targeted integration at the TCR locus and simultaneous KO of B2M. ( A ) Schematic of experimental design in primary T cells. An eGFP-expressing 4.4 kb cargo was integrated into the TRAC locus using an ssODN containing an attP site as an HDR template, together with a <t>sgRNA</t> targeting the TRAC locus. A B2M-targeting guide RNA can be co-delivered to achieve simultaneous KO at the B2M locus. Created in BioRender. Bassett, A. (2025) https://BioRender.com/lwtgerr . ( B ). Left: Bar chart showing ∼12% tagging efficiency at the TRAC locus in primary T cells. Data represent triplicate experiments; error bars indicate standard deviation. Data were analysed using two-sided t test. Right: Representative flow cytometry plot illustrating successful eGFP integration. ( C ) Schematic and flow cytometry analysis of simultaneous eGFP integration at the TRAC locus and B2M KO in primary T cells. All components were delivered in a <t>single</t> <t>nucleofection.</t> Seven days post-nucleofection, flow cytometry analysis revealed that >90% of eGFP-positive cells (indicating successful TRAC targeting) lacked B2M expression, compared to ∼30% B2M KO in the total cell population. Error bars indicate standard deviation. Data were analysed using two-sided t test.
Gene Targeting, supplied by Synthego Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/p3+primary+cell+nucleofector+solution+buffer/gene+targeting/pmc12956571-275-15-22
Average 86 stars, based on 1 article reviews
gene targeting - by Bioz Stars, 2026-10
86/100 stars
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aavs1 talen r  (Addgene inc)
95
Addgene inc aavs1 talen r
Targeted integration at the TCR locus and simultaneous KO of B2M. ( A ) Schematic of experimental design in primary T cells. An eGFP-expressing 4.4 kb cargo was integrated into the TRAC locus using an ssODN containing an attP site as an HDR template, together with a <t>sgRNA</t> targeting the TRAC locus. A B2M-targeting guide RNA can be co-delivered to achieve simultaneous KO at the B2M locus. Created in BioRender. Bassett, A. (2025) https://BioRender.com/lwtgerr . ( B ). Left: Bar chart showing ∼12% tagging efficiency at the TRAC locus in primary T cells. Data represent triplicate experiments; error bars indicate standard deviation. Data were analysed using two-sided t test. Right: Representative flow cytometry plot illustrating successful eGFP integration. ( C ) Schematic and flow cytometry analysis of simultaneous eGFP integration at the TRAC locus and B2M KO in primary T cells. All components were delivered in a <t>single</t> <t>nucleofection.</t> Seven days post-nucleofection, flow cytometry analysis revealed that >90% of eGFP-positive cells (indicating successful TRAC targeting) lacked B2M expression, compared to ∼30% B2M KO in the total cell population. Error bars indicate standard deviation. Data were analysed using two-sided t test.
Aavs1 Talen R, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/p3+primary+cell+nucleofector+solution+buffer/AAVS1-TALEN-L+(Plasmid+%2359025)/pmc09561290-171-25-31
Average 95 stars, based on 1 article reviews
aavs1 talen r - by Bioz Stars, 2026-10
95/100 stars
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prepared 34 electroporation buffer  (Fisher Scientific)
86
Fisher Scientific prepared 34 electroporation buffer
Targeted integration at the TCR locus and simultaneous KO of B2M. ( A ) Schematic of experimental design in primary T cells. An eGFP-expressing 4.4 kb cargo was integrated into the TRAC locus using an ssODN containing an attP site as an HDR template, together with a <t>sgRNA</t> targeting the TRAC locus. A B2M-targeting guide RNA can be co-delivered to achieve simultaneous KO at the B2M locus. Created in BioRender. Bassett, A. (2025) https://BioRender.com/lwtgerr . ( B ). Left: Bar chart showing ∼12% tagging efficiency at the TRAC locus in primary T cells. Data represent triplicate experiments; error bars indicate standard deviation. Data were analysed using two-sided t test. Right: Representative flow cytometry plot illustrating successful eGFP integration. ( C ) Schematic and flow cytometry analysis of simultaneous eGFP integration at the TRAC locus and B2M KO in primary T cells. All components were delivered in a <t>single</t> <t>nucleofection.</t> Seven days post-nucleofection, flow cytometry analysis revealed that >90% of eGFP-positive cells (indicating successful TRAC targeting) lacked B2M expression, compared to ∼30% B2M KO in the total cell population. Error bars indicate standard deviation. Data were analysed using two-sided t test.
Prepared 34 Electroporation Buffer, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/p3+primary+cell+nucleofector+solution+buffer/34+buffer+electroporation+prepared/10__1158_slash_2159___8290__cd___25___1187-211-6-29
Average 86 stars, based on 1 article reviews
prepared 34 electroporation buffer - by Bioz Stars, 2026-10
86/100 stars
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4d nucleofection system  (Amaxa)
86
Amaxa 4d nucleofection system
Targeted integration at the TCR locus and simultaneous KO of B2M. ( A ) Schematic of experimental design in primary T cells. An eGFP-expressing 4.4 kb cargo was integrated into the TRAC locus using an ssODN containing an attP site as an HDR template, together with a <t>sgRNA</t> targeting the TRAC locus. A B2M-targeting guide RNA can be co-delivered to achieve simultaneous KO at the B2M locus. Created in BioRender. Bassett, A. (2025) https://BioRender.com/lwtgerr . ( B ). Left: Bar chart showing ∼12% tagging efficiency at the TRAC locus in primary T cells. Data represent triplicate experiments; error bars indicate standard deviation. Data were analysed using two-sided t test. Right: Representative flow cytometry plot illustrating successful eGFP integration. ( C ) Schematic and flow cytometry analysis of simultaneous eGFP integration at the TRAC locus and B2M KO in primary T cells. All components were delivered in a <t>single</t> <t>nucleofection.</t> Seven days post-nucleofection, flow cytometry analysis revealed that >90% of eGFP-positive cells (indicating successful TRAC targeting) lacked B2M expression, compared to ∼30% B2M KO in the total cell population. Error bars indicate standard deviation. Data were analysed using two-sided t test.
4d Nucleofection System, supplied by Amaxa, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/p3+primary+cell+nucleofector+solution+buffer/4d+amaxa+nucleofection+system/10__1038_slash_s41596___018___0069___7-384-5-4
Average 86 stars, based on 1 article reviews
4d nucleofection system - by Bioz Stars, 2026-10
86/100 stars
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guide rna  (Synthego Inc)
86
Synthego Inc guide rna
Targeted integration at the TCR locus and simultaneous KO of B2M. ( A ) Schematic of experimental design in primary T cells. An eGFP-expressing 4.4 kb cargo was integrated into the TRAC locus using an ssODN containing an attP site as an HDR template, together with a <t>sgRNA</t> targeting the TRAC locus. A B2M-targeting guide RNA can be co-delivered to achieve simultaneous KO at the B2M locus. Created in BioRender. Bassett, A. (2025) https://BioRender.com/lwtgerr . ( B ). Left: Bar chart showing ∼12% tagging efficiency at the TRAC locus in primary T cells. Data represent triplicate experiments; error bars indicate standard deviation. Data were analysed using two-sided t test. Right: Representative flow cytometry plot illustrating successful eGFP integration. ( C ) Schematic and flow cytometry analysis of simultaneous eGFP integration at the TRAC locus and B2M KO in primary T cells. All components were delivered in a <t>single</t> <t>nucleofection.</t> Seven days post-nucleofection, flow cytometry analysis revealed that >90% of eGFP-positive cells (indicating successful TRAC targeting) lacked B2M expression, compared to ∼30% B2M KO in the total cell population. Error bars indicate standard deviation. Data were analysed using two-sided t test.
Guide Rna, supplied by Synthego Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/p3+primary+cell+nucleofector+solution+buffer/guide+rna/bio_rxiv__2025__08__14__670174-105-29-35
Average 86 stars, based on 1 article reviews
guide rna - by Bioz Stars, 2026-10
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Image Search Results


Targeted integration at the TCR locus and simultaneous KO of B2M. ( A ) Schematic of experimental design in primary T cells. An eGFP-expressing 4.4 kb cargo was integrated into the TRAC locus using an ssODN containing an attP site as an HDR template, together with a sgRNA targeting the TRAC locus. A B2M-targeting guide RNA can be co-delivered to achieve simultaneous KO at the B2M locus. Created in BioRender. Bassett, A. (2025) https://BioRender.com/lwtgerr . ( B ). Left: Bar chart showing ∼12% tagging efficiency at the TRAC locus in primary T cells. Data represent triplicate experiments; error bars indicate standard deviation. Data were analysed using two-sided t test. Right: Representative flow cytometry plot illustrating successful eGFP integration. ( C ) Schematic and flow cytometry analysis of simultaneous eGFP integration at the TRAC locus and B2M KO in primary T cells. All components were delivered in a single nucleofection. Seven days post-nucleofection, flow cytometry analysis revealed that >90% of eGFP-positive cells (indicating successful TRAC targeting) lacked B2M expression, compared to ∼30% B2M KO in the total cell population. Error bars indicate standard deviation. Data were analysed using two-sided t test.

Journal: Nucleic Acids Research

Article Title: ONE-STEP tagging: a versatile method for rapid site-specific integration by simultaneous reagent delivery

doi: 10.1093/nar/gkaf809

Figure Lengend Snippet: Targeted integration at the TCR locus and simultaneous KO of B2M. ( A ) Schematic of experimental design in primary T cells. An eGFP-expressing 4.4 kb cargo was integrated into the TRAC locus using an ssODN containing an attP site as an HDR template, together with a sgRNA targeting the TRAC locus. A B2M-targeting guide RNA can be co-delivered to achieve simultaneous KO at the B2M locus. Created in BioRender. Bassett, A. (2025) https://BioRender.com/lwtgerr . ( B ). Left: Bar chart showing ∼12% tagging efficiency at the TRAC locus in primary T cells. Data represent triplicate experiments; error bars indicate standard deviation. Data were analysed using two-sided t test. Right: Representative flow cytometry plot illustrating successful eGFP integration. ( C ) Schematic and flow cytometry analysis of simultaneous eGFP integration at the TRAC locus and B2M KO in primary T cells. All components were delivered in a single nucleofection. Seven days post-nucleofection, flow cytometry analysis revealed that >90% of eGFP-positive cells (indicating successful TRAC targeting) lacked B2M expression, compared to ∼30% B2M KO in the total cell population. Error bars indicate standard deviation. Data were analysed using two-sided t test.

Article Snippet: Nucleofection of sgRNA (Synthego), Cas9 protein (produced in-house or HiFi Cas9, IDT), and ssODN (Ultramer DNA oligo, IDT) into the hiPSC BFP reporter line was carried out in 100 μl cuvettes (Lonza) using an Amaxa 4D-Nucleofector (Lonza), P3 Primary Cell buffer, and program CA137.

Techniques: Expressing, Standard Deviation, Flow Cytometry